Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G
Sigma Aldrich Fine Chemicals Biosciences MES Hydrate | 1266615-59-1 | MFCD00149409 | 1kg
MES Hydrate | Purity: 99% | Mol Wt: 195.24 (anhydrous basis) | 1266615-59-1 | MFCD00149409 | 1kg
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
Rockland Immunochemicals 1X SDS-PAGE Destain Solution (Ready-to Use) - MB-022-1000
Destain Solution is a uniquely formulated, ready-to-use reagent specifically designed for in-gel visualization of Coomassie Blue stained total protein in polyacrylamide gels. The proprietary formulation of the solution ensures high detection sensitivity and low backgrounds.
Rockland Immunochemicals 1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED - MB-058
1X TES pH 7.8 DEPC TREATED - TES buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TES buffer is useful to stabilize proteins, DNA, or RNA, while protecting it from degradation.
Rockland Immunochemicals 1.0 M Tris HCl pH 9.0 - MB-028-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Aniara Diagnostica LLC Phosphate Buffered Saline, pH 7.4, 100 tablets
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 500 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
Kd Medical Inc TRIS BUFFER, 2M, PH 8.0, 1L
Buffer used in a wide range of Molecular Biology applications. 2 Molar solution pH 8.0. One 1 Liter bottle.
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 1KG
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 1KG
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt | 76836-02-7 | MFCD00064349 | 100 g
PIPES disodium salt | Purity: ≥99% | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 100 g
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Gold Biotechnology Inc Bis-Tris 500 g
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
Kd Medical Inc TRIS BUFF HCL PH8.0 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 8.0. One 1 liter bottle.