Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services TRIS HCL GMP GRADE 50KG
NC3654763 TRIS HCL GMP GRADE 50KG
Hoefer, Inc MOPS-SDS RUNNING BUFFER 1LX10
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cmWells: 12-well, 15-well Concentration:• Gradient gels: 4-12ĉĉĉĉĉĉĉĉĉĉMOPS-SDS Running Buffer, 1L×10ĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Hoefer, Inc MSML PRECSTGEL 10 BIS-TRIS 12W
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.#11;Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cm#11;Wells: 12-well, 15-well Concentration:#11;• Gradient gels: 4-12%ĉĉĉĉĉĉĉĉĉĉMighty Small precast gels, 10% Bis-Tris, 12wellsĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 25g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 25g
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 500g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 500g
IBA LifeSciences Buffer XT-R - Strep-TactinXT regeneration buffer; 250 ml
Buffer XT-R is a ready-to-use Strep-TactinXT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-TactinXT after the elution of Strep-tagII or Twin-Strep-tag fusion proteins. Please note that Buffer XT-R is not applicable for Strep-Tactin.
Kd Medical Inc PBS, 10X, PH7.4, 1 L BOTTLE
10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 1 Liter bottle.
Kd Medical Inc TRIS BASE, 1M, 1L BTL
Common buffered used in microbiological applications. 1 Molar solution. One 1 liter bottle.
Growcells TRIS-EDTA PH8 1L EA
Ready to use, sterile TE (Tris-EDTA) buffer solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. pH 8.0
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
STEMCELL Technologies HBSS with 10 mM HEPES, Without Phenol Red, Size: 500 mL
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating, or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.